Journal: iScience

Article Title: Downregulation of protein kinase C gamma reduces epithelial property and enhances malignant phenotypes in colorectal cancer cells

doi: 10.1016/j.isci.2022.105501

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal Fibronectin , Merck , Cat#Ab1954, RRID: AB_11213226.

Techniques: Recombinant, Reporter Assay, Expressing, Negative Control, Plasmid Preparation, Software

Journal: Molecular Vision

Article Title: Downregulation of LIM kinase 1 suppresses ocular inflammation and fibrosis

doi:

Figure Lengend Snippet: LIMK1 siRNA downregulates LIMK1 protein and suppresses fibronectin deposition in human corneal fibroblasts. A : western blot analysis. Corneal fibroblasts were transfected as indicated for 72 h. Cells were lysed and protein levels were analyzed using LIMK1 and GAPGH antibodies. B-E : Immunofluorescence microscopy using fibronectin antibody and DAPI staining in fibroblast cultures ( B ) untreated; ( C ) treated with 100 nM scrambled control siRNA; ( D ) treated with 50 nM of LIMK1 -targeted siRNA; or ( E ) treated with 100 nM of LIMK1 -targeted siRNA. F : Quantitative analysis of fibronectin secreted to the culture media by western blotting. Samples were untreated or treated with control or LIMK1 -targeted siRNA as indicated. Experiments were performed 3 times yielding similar results. Error bars represent standard deviation. The asterisk indicates a p<0.025 (n=3) compared to scrambled siRNA.

Article Snippet: To carry out immunostaining, corneal fibroblasts were incubated with polyclonal rabbit anti-human fibronectin (1:100; BD Biosciences, Lexington, KY) or monoclonal anti-paxillin (1:100; Millipore, Billercia, MA).

Techniques: Western Blot, Transfection, Immunofluorescence, Microscopy, Staining, Standard Deviation